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Metabolic & Endocrine · Clinical trials

MASH / NASH (Metabolic Steatohepatitis) Clinical Trials

Approved therapies, the pivotal-trial endpoints they were judged on, the magnitude of benefit — and the drugs that failed their endpoints, and why.

Indication overview

About mash / nash (metabolic steatohepatitis) — and why its trials are hard

Metabolic dysfunction-associated steatohepatitis (MASH, formerly nonalcoholic steatohepatitis, NASH) is the progressive, inflammatory form of fatty liver disease, marked by hepatic steatosis, lobular inflammation, hepatocyte ballooning, and fibrosis that can advance to cirrhosis and hepatocellular carcinoma. Tightly linked to obesity, type 2 diabetes, and metabolic syndrome, it is a leading and rising cause of liver transplantation. For years there was no approved pharmacotherapy, and the field accumulated a notable failure graveyard: obeticholic acid (REGENERATE) was twice rejected by the FDA over a benefit-risk concern; elafibranor (RESOLVE-IT), selonsertib (STELLAR), and cenicriviroc (AURORA) all missed their pivotal histologic endpoints. The breakthrough came in March 2024 when resmetirom (Rezdiffra), a liver-directed thyroid hormone receptor-beta agonist, became the first FDA-approved MASH therapy, based on MAESTRO-NASH meeting both co-primary histologic endpoints. Management still centers on weight loss and cardiometabolic risk control, with incretin-based agents (semaglutide, tirzepatide) showing strong resolution signals in trials, positioning MASH as a rapidly evolving therapeutic area built on dual histologic outcomes.

Indication
MASH / NASH (Metabolic Steatohepatitis)
ICD-10-CM
K75.81 — Nonalcoholic steatohepatitis

Approved therapies & pivotal evidence

What's been approved — and by how much it moved the endpoint

Drug (brand)ApprovedSettingPivotal trialPrimary endpointMagnitude of benefit
Resmetirom (Rezdiffra)2024First approved therapy for noncirrhotic MASH with moderate-to-advanced (F2-F3) fibrosis, with diet and exerciseMAESTRO-NASH Phase 3 (NCT03900429; NEJM 2024)Co-primary: NASH resolution without worsening fibrosis, and >=1-stage fibrosis improvement without worsening NAS, at week 52NASH resolution ~26-30% and fibrosis improvement ~24-26% versus ~10-14% placebo (both co-primary endpoints met)

Where trials have failed

Drugs that missed their endpoint — and what contributed

The most instructive lessons in mash / nash (metabolic steatohepatitis) development come from programmes that failed the endpoint that mattered.

Drug / trialEndpoint outcomeWhat contributed
Obeticholic acid — REGENERATE Phase 3 (FXR agonist)Met fibrosis-improvement endpoint on interim analysis but twice rejected by the FDA (2020 CRL; second complete response letter June 2023)Unfavorable benefit-risk balance: modest histologic benefit against pruritus, dyslipidemia (LDL rise), and gallbladder/hepatic safety concerns
Elafibranor — RESOLVE-IT Phase 3 (PPAR-alpha/delta agonist)Failed to meet primary endpoint of NASH resolution without worsening fibrosis; program discontinued in NASHInsufficient histologic efficacy; response rates not significantly better than placebo (elafibranor later approved in PBC instead)
Selonsertib — STELLAR-3 / STELLAR-4 Phase 3 (ASK1 inhibitor)Failed to meet primary fibrosis-improvement endpoint in bridging fibrosis and cirrhosisNo antifibrotic benefit versus placebo; targeting a single stress-kinase pathway proved inadequate
Cenicriviroc — AURORA Phase 3 (CCR2/CCR5 antagonist)Terminated after interim analysis showed lack of efficacy on fibrosis improvementAnti-inflammatory/antifibrotic mechanism did not translate into histologic benefit

Choosing the right endpoint

Primary endpoints that matter in mash / nash (metabolic steatohepatitis) trials

  • NASH/MASH resolution without worsening of fibrosis — Histologic co-primary reflecting reversal of steatohepatitis (ballooning + inflammation)
  • Fibrosis improvement (>=1 stage) without worsening of steatohepatitis — Histologic co-primary capturing the prognostically critical fibrosis component
  • NAFLD Activity Score (NAS) components — Semiquantitative scoring of steatosis, ballooning, and inflammation on biopsy
  • Noninvasive markers (MRI-PDFF, liver stiffness, ALT) — Supportive/secondary measures reducing reliance on serial biopsy

How iNGENū runs mash / nash (metabolic steatohepatitis) trials

Physician-led design, built for FDA submission

Endpoint & biomarker strategy

Board-certified specialists design endpoints and patient selection aligned to current FDA guidance for this indication.

FDA-ready data

Built to ICH-GCP and 21 CFR 312.120, with direct FDA submission — data accepted by the FDA, EMA and MHRA.

Faster, lower-cost delivery

~4-week ethics via the TGA CTN scheme, up to 43.5% R&D rebate, and 80–90% below US CRO cost.

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Frequently asked questions

MASH / NASH (Metabolic Steatohepatitis) clinical trials — FAQs

What changed with resmetirom's approval?
In March 2024 resmetirom (Rezdiffra) became the first FDA-approved drug for MASH with moderate-to-advanced fibrosis, ending an era with no approved pharmacotherapy. In MAESTRO-NASH it met both co-primary histologic endpoints: NASH resolution and fibrosis improvement, each significantly better than placebo.
Why did obeticholic acid fail to gain approval?
Despite meeting a fibrosis endpoint in REGENERATE, obeticholic acid was rejected twice by the FDA. Regulators judged the modest histologic benefit insufficient against safety concerns including intense pruritus, LDL-cholesterol elevation, and hepatobiliary risks, an unfavorable benefit-risk balance in a chronic disease.
Do lifestyle changes and diabetes drugs still matter?
Yes. Weight loss and cardiometabolic risk control remain foundational, and clinically meaningful weight reduction can improve liver histology. Incretin therapies such as semaglutide and tirzepatide have shown strong MASH resolution signals in trials, making weight-centric management complementary to resmetirom.

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