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Cardiovascular · Clinical trials

ATTR Cardiac Amyloidosis Clinical Trials

Approved therapies, the pivotal-trial endpoints they were judged on, the magnitude of benefit — and the drugs that failed their endpoints, and why.

Indication overview

About attr cardiac amyloidosis — and why its trials are hard

Transthyretin amyloid cardiomyopathy (ATTR-CM) is caused by the misfolding of transthyretin (TTR), a liver-produced tetrameric transport protein, into amyloid fibrils that deposit in the myocardium and stiffen the heart, producing a restrictive cardiomyopathy and progressive heart failure with preserved ejection fraction. It occurs in a wild-type form (age-related, predominantly older men) and a hereditary form from TTR gene mutations. Once considered rare and untreatable, ATTR-CM is now increasingly diagnosed noninvasively using technetium pyrophosphate scintigraphy with exclusion of a plasma cell dyscrasia. Disease-modifying therapy targets the TTR protein through two strategies: stabilizers that hold the tetramer together to prevent fibril formation, and gene silencers (siRNA) that reduce hepatic TTR production. Tafamidis was the first approved stabilizer, followed by acoramidis, a near-complete stabilizer. The RNA-interference agents vutrisiran and patisiran suppress TTR synthesis; vutrisiran gained an ATTR-CM indication after the HELIOS-B trial. These therapies reduce cardiovascular death and hospitalization and slow functional decline, though they do not clear existing amyloid deposits, making early diagnosis and treatment critical.

Indication
ATTR Cardiac Amyloidosis
ICD-10-CM
E85.82 — Wild-type transthyretin amyloidosis

Approved therapies & pivotal evidence

What's been approved — and by how much it moved the endpoint

Drug (brand)ApprovedSettingPivotal trialPrimary endpointMagnitude of benefit
Tafamidis (Vyndaqel / Vyndamax)2019Wild-type or hereditary ATTR-CM (first-approved TTR stabilizer)ATTR-ACT (NEJM 2018)Hierarchical composite of all-cause mortality and frequency of cardiovascular-related hospitalizations over 30 monthsAll-cause mortality 29.5% vs 42.9% with placebo (HR 0.70); reduced CV hospitalizations (0.48 vs 0.70 per year) and slowed decline in 6MWD and KCCQ
Acoramidis (Attruby)2024Wild-type or hereditary ATTR-CM (near-complete TTR stabilizer, >=90%)ATTRibute-CM (NEJM 2024)Hierarchical outcome of all-cause death, cardiovascular hospitalization, change in NT-proBNP, and change in 6-minute walk distance at 30 monthsWin ratio 1.8 favoring acoramidis (p<0.001); reduced cumulative CV-related hospitalizations and NT-proBNP versus placebo
Vutrisiran (Amvuttra)2025 (ATTR-CM indication; earlier approved 2022 for hATTR polyneuropathy)ATTR-CM to reduce cardiovascular death and events (RNAi TTR silencer)HELIOS-B (NEJM 2025)Composite of all-cause mortality and recurrent cardiovascular eventsSignificant reduction in the composite (HR approximately 0.72 in the overall population) with reductions in all-cause mortality and CV events; first RNAi therapeutic with an ATTR-CM cardiovascular outcome benefit
Patisiran (Onpattro)2018 (hATTR polyneuropathy; NOT approved for ATTR-CM)Hereditary ATTR with polyneuropathy (RNAi TTR silencer)APOLLO (NEJM 2018); APOLLO-B (cardiomyopathy, 2023)APOLLO: modified Neuropathy Impairment Score +7 (mNIS+7); APOLLO-B: 6-minute walk distanceAPOLLO: significant improvement in neuropathy vs placebo; APOLLO-B showed a 6MWD benefit but FDA declined a cardiomyopathy indication (see failed)

Where trials have failed

Drugs that missed their endpoint — and what contributed

The most instructive lessons in attr cardiac amyloidosis development come from programmes that failed the endpoint that mattered.

Drug / trialEndpoint outcomeWhat contributed
Patisiran (for the cardiomyopathy indication) — APOLLO-B (2023)Although APOLLO-B met its primary 6-minute walk distance endpoint, the FDA declined to approve patisiran for ATTR-CM, concluding the demonstrated effect was not clinically meaningful enough for a cardiomyopathy indicationModest functional benefit without a convincing mortality/CV outcome signal at review; the ATTR-CM indication went to stabilizers and later to vutrisiran
Revusiran — ENDEAVOURThe first-generation RNAi agent revusiran was discontinued in 2016 after an imbalance in deaths in the treatment armSafety/mortality signal halted development; the program pivoted to the enhanced-stabilization chemistry siRNAs patisiran and vutrisiran

Choosing the right endpoint

Primary endpoints that matter in attr cardiac amyloidosis trials

  • All-cause mortality — Hard outcome and the top tier of the hierarchical composites in ATTR-ACT and ATTRibute-CM
  • Cardiovascular hospitalization — Frequency of CV-related admissions; second tier of the pivotal stabilizer composites
  • Composite of death and recurrent CV events — Primary endpoint in HELIOS-B for vutrisiran
  • 6-minute walk distance (6MWD) — Functional capacity measure used across ATTR-CM trials, including the APOLLO-B primary endpoint
  • NT-proBNP and KCCQ — Biomarker of cardiac stress and quality-of-life instrument used as key secondary/hierarchical endpoints

How iNGENū runs attr cardiac amyloidosis trials

Physician-led design, built for FDA submission

Endpoint & biomarker strategy

Board-certified specialists design endpoints and patient selection aligned to current FDA guidance for this indication.

FDA-ready data

Built to ICH-GCP and 21 CFR 312.120, with direct FDA submission — data accepted by the FDA, EMA and MHRA.

Faster, lower-cost delivery

~4-week ethics via the TGA CTN scheme, up to 43.5% R&D rebate, and 80–90% below US CRO cost.

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Frequently asked questions

ATTR Cardiac Amyloidosis clinical trials — FAQs

Do ATTR-CM drugs remove amyloid already in the heart?
Generally no. Stabilizers (tafamidis, acoramidis) prevent new fibril formation and silencers (vutrisiran, patisiran) cut TTR production, slowing progression and reducing events, but they do not clear existing deposits. This makes early diagnosis and treatment important. Investigational antibody depleters aiming to remove amyloid are in development.
What is the difference between a TTR stabilizer and a TTR silencer?
Stabilizers such as tafamidis and acoramidis bind the transthyretin tetramer to keep it from dissociating and misfolding. Silencers such as vutrisiran and patisiran are RNA-interference drugs that reduce the liver's production of transthyretin. Both reduce cardiovascular events in ATTR-CM.
Why is tafamidis considered a landmark therapy?
ATTR-ACT was the first trial to show a disease-modifying drug reduced all-cause mortality and cardiovascular hospitalizations in ATTR-CM, transforming a formerly untreatable, uniformly progressive disease. Its 2019 FDA approval established TTR stabilization as a viable therapeutic strategy.

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